Cell Culture:Article Title: Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis
Article Snippet: .. U2-OS (ATCC HTB-96), A549 (ATCC CCL-185), and NIH3T3 (ATCC CRL-1658) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) media containing 10% FBS (Hyclone, SH30071.03) and 1% Pen/Strep (Gibco, 15140-122) at 37°C with 5% CO 2 . .. Cells were dissociated with TrypLE Express (Thermo Fisher Scientific, 12605010) for passaging.
Modification:Article Title: Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis
Article Snippet: .. U2-OS (ATCC HTB-96), A549 (ATCC CCL-185), and NIH3T3 (ATCC CRL-1658) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) media containing 10% FBS (Hyclone, SH30071.03) and 1% Pen/Strep (Gibco, 15140-122) at 37°C with 5% CO 2 . .. Cells were dissociated with TrypLE Express (Thermo Fisher Scientific, 12605010) for passaging.
In Vitro:Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells.
Article Snippet: .. In vitro assays were conducted using OS cell lines SAOS- 2 and U2- OS (ATCC, Cat. no. HTB- 85; Cat. no. HTB- 96) [42], along with human bone marrow- derived mesenchymal stem cells (hBMSCs) (Lonza Milan, Italy, PT- 2501). hBMSCs were characterised by flow cytometry analysis (FCA) for MSC surface markers, including positive markers (CD29, CD73 and CD90) and negative markers (CD14 and CD45) [43]. .. Cells were maintained at 37°C in a humidified atmosphere with 5% CO2 in DMEM/F12 medium (Euroclone, Milan, Italy), supplemented with 10% FBS (Cat. no. ECS0180L, Euroclone, Milan, Italy) and 2% penicillin/ streptomycin (P/S) (Lonza, Milan, Italy).
Article Title: Gene Expression Alterations Associated With Resveratrol‐Induced Antiproliferative Effects and S‐Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells
Article Snippet: .. In vitro assays were conducted using OS cell lines SAOS‐2 and U2‐OS (ATCC, Cat. no. HTB‐85; Cat. no. HTB‐96) [ ], along with human bone marrow‐derived mesenchymal stem cells (hBMSCs) (Lonza Milan, Italy, PT‐2501). hBMSCs were characterised by flow cytometry analysis (FCA) for MSC surface markers, including positive markers (CD29, CD73 and CD90) and negative markers (CD14 and CD45) [ ]. .. Cells were maintained at 37°C in a humidified atmosphere with 5% CO 2 in DMEM/F12 medium (Euroclone, Milan, Italy), supplemented with 10% FBS (Cat. no. ECS0180L, Euroclone, Milan, Italy) and 2% penicillin/streptomycin (P/S) (Lonza, Milan, Italy).
Derivative Assay:Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells.
Article Snippet: .. In vitro assays were conducted using OS cell lines SAOS- 2 and U2- OS (ATCC, Cat. no. HTB- 85; Cat. no. HTB- 96) [42], along with human bone marrow- derived mesenchymal stem cells (hBMSCs) (Lonza Milan, Italy, PT- 2501). hBMSCs were characterised by flow cytometry analysis (FCA) for MSC surface markers, including positive markers (CD29, CD73 and CD90) and negative markers (CD14 and CD45) [43]. .. Cells were maintained at 37°C in a humidified atmosphere with 5% CO2 in DMEM/F12 medium (Euroclone, Milan, Italy), supplemented with 10% FBS (Cat. no. ECS0180L, Euroclone, Milan, Italy) and 2% penicillin/ streptomycin (P/S) (Lonza, Milan, Italy).
Flow Cytometry:Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells.
Article Snippet: .. In vitro assays were conducted using OS cell lines SAOS- 2 and U2- OS (ATCC, Cat. no. HTB- 85; Cat. no. HTB- 96) [42], along with human bone marrow- derived mesenchymal stem cells (hBMSCs) (Lonza Milan, Italy, PT- 2501). hBMSCs were characterised by flow cytometry analysis (FCA) for MSC surface markers, including positive markers (CD29, CD73 and CD90) and negative markers (CD14 and CD45) [43]. .. Cells were maintained at 37°C in a humidified atmosphere with 5% CO2 in DMEM/F12 medium (Euroclone, Milan, Italy), supplemented with 10% FBS (Cat. no. ECS0180L, Euroclone, Milan, Italy) and 2% penicillin/ streptomycin (P/S) (Lonza, Milan, Italy).
Article Title: Gene Expression Alterations Associated With Resveratrol‐Induced Antiproliferative Effects and S‐Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells
Article Snippet: .. In vitro assays were conducted using OS cell lines SAOS‐2 and U2‐OS (ATCC, Cat. no. HTB‐85; Cat. no. HTB‐96) [ ], along with human bone marrow‐derived mesenchymal stem cells (hBMSCs) (Lonza Milan, Italy, PT‐2501). hBMSCs were characterised by flow cytometry analysis (FCA) for MSC surface markers, including positive markers (CD29, CD73 and CD90) and negative markers (CD14 and CD45) [ ]. .. Cells were maintained at 37°C in a humidified atmosphere with 5% CO 2 in DMEM/F12 medium (Euroclone, Milan, Italy), supplemented with 10% FBS (Cat. no. ECS0180L, Euroclone, Milan, Italy) and 2% penicillin/streptomycin (P/S) (Lonza, Milan, Italy).
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